strep tagged proteins (IBA Lifesciences)

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IBA Lifesciences strep tagged proteins
Strep Tagged Proteins, supplied by IBA Lifesciences, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/strep+tagged+proteins/Strep-Tactin/pm41381493-311-3-12
Average 99 stars, based on 1 article reviews

strep tagged proteins - by Bioz Stars, 2026-09

99/100 stars

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Centrifugation:

Article Title: An interbacterial cysteine protease toxin inhibits cell growth by targeting type II DNA topoisomerases GyrB and ParE
Article Snippet: .. Cellular debris was removed by centrifugation at 40,000 × g for 30 min. Strep-tagged proteins were purified from the supernatant using a 1 mL gravity-flow column containing streptavidin resin (IBA Lifesciences GmbH). ..

Purification:

Article Title: Evolutionary Remodelling of a Remnant GET-Pathway Factor into PEX38, a Novel and Essential Peroxin in Euglenozoa
Article Snippet: .. Purification of Strep-tagged proteins was performed according to the manufacturer’s protocol (Iba, cat. no. 2-5010-002). ..

Article Title: PexR is a noncanonical regulator of the peroxide stress response in bacteria
Article Snippet: .. Strep-tagged proteins were purified from the cell lysate supernatant off 1 ml Strep-Tactin XT4Flow resin (IBA Life Sciences) with 1 ml elutions using 50 mM biotin/buffer. .. Protein-containing fractions were pooled, concentrated (Amicon Ultra-15 MWCO 10 kDa), and purified using a SuperdexTM 200 Increase 10/300 GL SEC column and AKTA HPLC unit (Cytiva).

Article Title: Distinct roles of phytochromes A and B in Aspergillus fumigatus in environmental sensing and pathogenicity.
Article Snippet: .. The strep-tagged proteins were detected using StrepMAB-Classic HRPconjugated monoclonal antibodies (IBA Lifesciences, Göttingen, Germany). (Bottom) Zinc-induced red fluorescence of the purified phytochromes. ..

Article Title: Co-condensation between transcription factor and cBAF selectively modulates chromatin remodeling and gene expression.
Article Snippet: .. The Strep-GFP- or Strep-tagged proteins were purified using the Strep-Tactin Superflow beads (IBA). .. The His-mCherry-tagged proteins were purified using a Ni-NTA column (GE Healthcare).

Titration:

Article Title: S165F mutation-induced Junctophilin-2 autoinhibition impairs Ca 2+ signaling in hypertrophic cardiomyopathy.
Article Snippet: .. The titration data were analyzed by MicroCal PEAQ-ITC analysis 498 software (Malvern) and fitted by the one-site binding model. 499 500 Strep pull-down assay 501 Cell lysate-based Strep pull-down: Strep-tagged proteins were bound to 40 μL 502 Strep-Tactin XT beads (IBA Lifesciences) with 1 mg HEK293T lysate in RIPA buffer 503 (50 mM Tris pH 7.4, 150 mM NaCl, 1% Triton X-100) for 2 h at 4 °C, washed three 504 times, and eluted in SDS loading buffer. .. After centrifugation, supernatants containing 505 soluble proteins were resolved on a polyacrylamide gel with 10% SDS and analyzed by 506 Western blotting with anti-GFP antibody (Abcam, ab290) or anti-HA antibody (Abcam, 507 ab9110) at a 1/10000 dilution.

Software:

Binding Assay:

Pull Down Assay:

Bioprocessing:

Fluorescence:

other:

Article Title: A fin-loop-like structure in GPX4 underlies neuroprotection from ferroptosis.
Article Snippet: Imaging was carried out using the JEOL -1200 EXII transmission electron microscope (JEOL, Akish- ima, Tokyo) at 70 kV.

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